Xanthomonas axonopodis pv. punicae depends on multiple non-TAL (Xop) T3SS effectors for its coveted growth inside the pomegranate plant through repressing the immune responses during bacterial blight development.
Identifieur interne : 000003 ( Main/Exploration ); précédent : 000002; suivant : 000004Xanthomonas axonopodis pv. punicae depends on multiple non-TAL (Xop) T3SS effectors for its coveted growth inside the pomegranate plant through repressing the immune responses during bacterial blight development.
Auteurs : Kalyan K. Mondal [Inde] ; Madhvi Soni [Inde] ; Geeta Verma [Inde] ; Aditya Kulshreshtha [Inde] ; S. Mrutyunjaya [Inde] ; Rishikesh Kumar [Inde]Source :
- Microbiological research [ 1618-0623 ] ; 2020.
Abstract
Xanthomonas axonopodis pv. punicae (Xap), the bacterial blight pathogen of pomegranate, incurs substantial loss to yield and reduces export quality of this economically important fruit crop. During infection, the bacterium secretes six non-TAL (Xop) effectors into the pomegranate cells through a specialized type three secretion system (T3SS). Previously, we demonstrated the role of two key effectors, XopL and XopN in pathogenesis. Here, we investigate the role of rest effectors (XopC2, XopE1, XopQ and XopZ) on disease development. We generated null mutants for each individual effector and mutant bacterial suspension was infiltrated into pomegranate leaves. Compared to Xap wild, the mutant bacterial growth was reduced by 2.7-11.5 folds. The mutants produced lesser water-soaked lesions when infiltrated on leaves by 1.13-2.21 folds. Among the four effectors, XopC2 contributes highest for in planta bacterial growth and disease development. XopC2 efficiently suppressed the defense responses like callose deposition, reactive oxygen species (ROS) and the activation of immune responsive genes. Being a major contributor, we further characterize XopC2 for its subcellular localization, its protein structure and networking. XopC2 is localized to the plasma membrane of Nicotiana benthamiana like XopL and XopN. XopC2 is a 661 amino acids protein having 15 alpha and 17 beta helix. Our STRING and I-TASSER based analysis hinted that XopC2 interacts with multiple membrane localized plant proteins including transcription regulator of CCR4-NOT family, TTN of maintenance of chromosome family and serine/threonine-protein phosphatase 2A (PP2A) isoform. Based on the interaction it is predicted that XopC2 might involve in diverse functions like nuclear-transcribed mRNA catabolic process, maintenance of chromosome, hormone signaling and protein dephosphorylation activities and thereby suppress the plant immunity. Altogether, our study suggests that Xap largely depends on three non-TAL (Xop) effectors, including XopC2, XopL and XopN, to modulate pomegranate PTI for its unrestricted proliferation during bacterial blight development.
DOI: 10.1016/j.micres.2020.126560
PubMed: 32721820
Affiliations:
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Mondal</name><affiliation wicri:level="1"><nlm:affiliation>Plant Bacteriology Lab, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, Pusa Campus, New Delhi 110012, India. Electronic address: kalyan.mondal@icar.gov.in.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Plant Bacteriology Lab, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, Pusa Campus, New Delhi 110012</wicri:regionArea><wicri:noRegion>New Delhi 110012</wicri:noRegion></affiliation></author><author><name sortKey="Soni, Madhvi" sort="Soni, Madhvi" uniqKey="Soni M" first="Madhvi" last="Soni">Madhvi Soni</name><affiliation wicri:level="1"><nlm:affiliation>Plant Bacteriology Lab, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, Pusa Campus, New Delhi 110012, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Plant Bacteriology Lab, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, Pusa Campus, New Delhi 110012</wicri:regionArea><wicri:noRegion>New Delhi 110012</wicri:noRegion></affiliation></author><author><name sortKey="Verma, Geeta" sort="Verma, Geeta" uniqKey="Verma G" first="Geeta" last="Verma">Geeta Verma</name><affiliation wicri:level="1"><nlm:affiliation>Plant Bacteriology Lab, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, Pusa Campus, New Delhi 110012, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Plant Bacteriology Lab, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, Pusa Campus, New Delhi 110012</wicri:regionArea><wicri:noRegion>New Delhi 110012</wicri:noRegion></affiliation></author><author><name sortKey="Kulshreshtha, Aditya" sort="Kulshreshtha, Aditya" uniqKey="Kulshreshtha A" first="Aditya" last="Kulshreshtha">Aditya Kulshreshtha</name><affiliation wicri:level="1"><nlm:affiliation>Plant Bacteriology Lab, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, Pusa Campus, New Delhi 110012, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Plant Bacteriology Lab, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, Pusa Campus, New Delhi 110012</wicri:regionArea><wicri:noRegion>New Delhi 110012</wicri:noRegion></affiliation></author><author><name sortKey="Mrutyunjaya, S" sort="Mrutyunjaya, S" uniqKey="Mrutyunjaya S" first="S" last="Mrutyunjaya">S. Mrutyunjaya</name><affiliation wicri:level="1"><nlm:affiliation>Plant Bacteriology Lab, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, Pusa Campus, New Delhi 110012, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Plant Bacteriology Lab, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, Pusa Campus, New Delhi 110012</wicri:regionArea><wicri:noRegion>New Delhi 110012</wicri:noRegion></affiliation></author><author><name sortKey="Kumar, Rishikesh" sort="Kumar, Rishikesh" uniqKey="Kumar R" first="Rishikesh" last="Kumar">Rishikesh Kumar</name><affiliation wicri:level="1"><nlm:affiliation>ICAR-Indian Institute of Pulses Research, Kanpur, Uttar Pradesh 208024, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>ICAR-Indian Institute of Pulses Research, Kanpur, Uttar Pradesh 208024</wicri:regionArea><wicri:noRegion>Uttar Pradesh 208024</wicri:noRegion></affiliation></author></analytic><series><title level="j">Microbiological research</title><idno type="eISSN">1618-0623</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Xanthomonas axonopodis pv. punicae (Xap), the bacterial blight pathogen of pomegranate, incurs substantial loss to yield and reduces export quality of this economically important fruit crop. During infection, the bacterium secretes six non-TAL (Xop) effectors into the pomegranate cells through a specialized type three secretion system (T3SS). Previously, we demonstrated the role of two key effectors, XopL and XopN in pathogenesis. Here, we investigate the role of rest effectors (XopC2, XopE1, XopQ and XopZ) on disease development. We generated null mutants for each individual effector and mutant bacterial suspension was infiltrated into pomegranate leaves. Compared to Xap wild, the mutant bacterial growth was reduced by 2.7-11.5 folds. The mutants produced lesser water-soaked lesions when infiltrated on leaves by 1.13-2.21 folds. Among the four effectors, XopC2 contributes highest for in planta bacterial growth and disease development. XopC2 efficiently suppressed the defense responses like callose deposition, reactive oxygen species (ROS) and the activation of immune responsive genes. Being a major contributor, we further characterize XopC2 for its subcellular localization, its protein structure and networking. XopC2 is localized to the plasma membrane of Nicotiana benthamiana like XopL and XopN. XopC2 is a 661 amino acids protein having 15 alpha and 17 beta helix. Our STRING and I-TASSER based analysis hinted that XopC2 interacts with multiple membrane localized plant proteins including transcription regulator of CCR4-NOT family, TTN of maintenance of chromosome family and serine/threonine-protein phosphatase 2A (PP2A) isoform. Based on the interaction it is predicted that XopC2 might involve in diverse functions like nuclear-transcribed mRNA catabolic process, maintenance of chromosome, hormone signaling and protein dephosphorylation activities and thereby suppress the plant immunity. Altogether, our study suggests that Xap largely depends on three non-TAL (Xop) effectors, including XopC2, XopL and XopN, to modulate pomegranate PTI for its unrestricted proliferation during bacterial blight development.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32721820</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>16</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1618-0623</ISSN><JournalIssue CitedMedium="Internet"><Volume>240</Volume><PubDate><Year>2020</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Microbiological research</Title><ISOAbbreviation>Microbiol Res</ISOAbbreviation></Journal><ArticleTitle>Xanthomonas axonopodis pv. punicae depends on multiple non-TAL (Xop) T3SS effectors for its coveted growth inside the pomegranate plant through repressing the immune responses during bacterial blight development.</ArticleTitle><Pagination><MedlinePgn>126560</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0944-5013(20)30428-6</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.micres.2020.126560</ELocationID><Abstract><AbstractText>Xanthomonas axonopodis pv. punicae (Xap), the bacterial blight pathogen of pomegranate, incurs substantial loss to yield and reduces export quality of this economically important fruit crop. During infection, the bacterium secretes six non-TAL (Xop) effectors into the pomegranate cells through a specialized type three secretion system (T3SS). Previously, we demonstrated the role of two key effectors, XopL and XopN in pathogenesis. Here, we investigate the role of rest effectors (XopC2, XopE1, XopQ and XopZ) on disease development. We generated null mutants for each individual effector and mutant bacterial suspension was infiltrated into pomegranate leaves. Compared to Xap wild, the mutant bacterial growth was reduced by 2.7-11.5 folds. The mutants produced lesser water-soaked lesions when infiltrated on leaves by 1.13-2.21 folds. Among the four effectors, XopC2 contributes highest for in planta bacterial growth and disease development. XopC2 efficiently suppressed the defense responses like callose deposition, reactive oxygen species (ROS) and the activation of immune responsive genes. Being a major contributor, we further characterize XopC2 for its subcellular localization, its protein structure and networking. XopC2 is localized to the plasma membrane of Nicotiana benthamiana like XopL and XopN. XopC2 is a 661 amino acids protein having 15 alpha and 17 beta helix. Our STRING and I-TASSER based analysis hinted that XopC2 interacts with multiple membrane localized plant proteins including transcription regulator of CCR4-NOT family, TTN of maintenance of chromosome family and serine/threonine-protein phosphatase 2A (PP2A) isoform. Based on the interaction it is predicted that XopC2 might involve in diverse functions like nuclear-transcribed mRNA catabolic process, maintenance of chromosome, hormone signaling and protein dephosphorylation activities and thereby suppress the plant immunity. Altogether, our study suggests that Xap largely depends on three non-TAL (Xop) effectors, including XopC2, XopL and XopN, to modulate pomegranate PTI for its unrestricted proliferation during bacterial blight development.</AbstractText><CopyrightInformation>Copyright © 2020 Elsevier GmbH. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Mondal</LastName><ForeName>Kalyan K</ForeName><Initials>KK</Initials><AffiliationInfo><Affiliation>Plant Bacteriology Lab, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, Pusa Campus, New Delhi 110012, India. Electronic address: kalyan.mondal@icar.gov.in.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Soni</LastName><ForeName>Madhvi</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Plant Bacteriology Lab, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, Pusa Campus, New Delhi 110012, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Verma</LastName><ForeName>Geeta</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Plant Bacteriology Lab, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, Pusa Campus, New Delhi 110012, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kulshreshtha</LastName><ForeName>Aditya</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Plant Bacteriology Lab, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, Pusa Campus, New Delhi 110012, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mrutyunjaya</LastName><ForeName>S</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Plant Bacteriology Lab, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, Pusa Campus, New Delhi 110012, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kumar</LastName><ForeName>Rishikesh</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>ICAR-Indian Institute of Pulses Research, Kanpur, Uttar Pradesh 208024, India.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>07</Month><Day>22</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Microbiol Res</MedlineTA><NlmUniqueID>9437794</NlmUniqueID><ISSNLinking>0944-5013</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Callose deposition</Keyword><Keyword MajorTopicYN="N">Plant immune responses</Keyword><Keyword MajorTopicYN="N">Pomegranate</Keyword><Keyword MajorTopicYN="N">ROS production</Keyword><Keyword MajorTopicYN="N">Xanthomonas axonopodis pv. punicae</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>02</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>07</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>07</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>7</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>7</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>7</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32721820</ArticleId><ArticleId IdType="pii">S0944-5013(20)30428-6</ArticleId><ArticleId IdType="doi">10.1016/j.micres.2020.126560</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Inde</li></country></list><tree><country name="Inde"><noRegion><name sortKey="Mondal, Kalyan K" sort="Mondal, Kalyan K" uniqKey="Mondal K" first="Kalyan K" last="Mondal">Kalyan K. Mondal</name></noRegion><name sortKey="Kulshreshtha, Aditya" sort="Kulshreshtha, Aditya" uniqKey="Kulshreshtha A" first="Aditya" last="Kulshreshtha">Aditya Kulshreshtha</name><name sortKey="Kumar, Rishikesh" sort="Kumar, Rishikesh" uniqKey="Kumar R" first="Rishikesh" last="Kumar">Rishikesh Kumar</name><name sortKey="Mrutyunjaya, S" sort="Mrutyunjaya, S" uniqKey="Mrutyunjaya S" first="S" last="Mrutyunjaya">S. Mrutyunjaya</name><name sortKey="Soni, Madhvi" sort="Soni, Madhvi" uniqKey="Soni M" first="Madhvi" last="Soni">Madhvi Soni</name><name sortKey="Verma, Geeta" sort="Verma, Geeta" uniqKey="Verma G" first="Geeta" last="Verma">Geeta Verma</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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